FastQCFastQC Report
Wed 6 Oct 2021
H5G5CBGXK_n01_DO01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5G5CBGXK_n01_DO01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19631522
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC8300844.228322185106178TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGG296780.15117523745739123TruSeq Adapter, Index 11 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATTC278760.1419961223587249TruSeq Adapter, Index 11 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)