FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n02_hbepc_b2_12h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n02_hbepc_b2_12h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43808195
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG314550.044.46043850
ATAGGGG159150.038.36598649
TATAGGG154800.036.9928148
GGGGAAT333800.032.49329463
GGGGGGT975800.031.86824614
GTTGGGA313950.031.14639519
GAATTAA256000.030.4825466
GGGAATT261350.029.51534364
GGGGTTG531900.029.24948316
GGAATTA214050.028.92995565
GGGGGTT534000.028.90184615
GGGTTGG546050.028.57999217
GGTTGGG557750.027.63381418
GTTTGGT360100.026.05036731
GGGGGAG1001350.024.7333243
TTATAGG183550.024.45136547
GGGAAAA736350.023.9004622
GTAGGGG224600.023.6839649
TTGGGAA418550.023.58419620
GGGGAGA672900.023.5676654
Produced by FastQC (version 0.11.2)