FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n02_hbepc_b2_12h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n02_hbepc_b2_12h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45566059
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG291300.046.22081850
ATAGGGG180000.042.20116449
TATAGGG185200.039.95451748
GGGGGTT432900.034.4158915
GGGGGGT732950.034.07140714
GTTGGGA345750.033.58058519
GGGTTGG468600.032.2725417
GGGGTTG466800.032.26393516
GGTTGGG484800.030.80939718
GGGGAAT299300.030.4069263
TTATAGG218200.029.50098247
GAATTAA253500.027.53104866
GGGGAGA642350.026.004524
GGGGGAG811800.025.532363
TTGGGAA471500.025.26036820
GGAATTA202050.025.08232365
GGGAAAA799050.025.082222
GGGAATT246150.024.00835464
GGGAGAG665650.023.5237065
GGAAAAA786500.023.1267923
Produced by FastQC (version 0.11.2)