FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n02_hbepc_b1_24h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n02_hbepc_b1_24h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43940678
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG252400.042.25964750
ATAGGGG182500.038.65504549
TATAGGG177800.038.5516848
TTATAGG199050.030.3113247
GGGTTGG381300.029.93363217
GGGGGGT663000.029.39179214
GTTGGGA302300.028.80792419
GGGGTTG405800.028.1859216
GGGGGTT405550.027.85447715
GGTTGGG416200.027.27401718
GAATTAA233050.023.59625466
GGGAAAA686950.023.3240322
TAAGGGG184050.022.5478750
TTTATAG273450.021.84978546
TTGGGAA412750.021.84246820
GGGGGAG757500.021.4238723
GGGGAGA583150.021.338574
GGGGGAT322700.021.07284534
GGAATTA202100.020.3988465
TATAAGG126400.020.3868848
Produced by FastQC (version 0.11.2)