FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n02_hbepc_b1_24h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n02_hbepc_b1_24h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38511957
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG170400.031.70284750
ATAGGGG99850.023.56201649
TATAGGG96150.022.24431848
GGGGGGT827100.017.70385714
GGGTTGG296450.017.49340817
GGTTGGG314750.016.42133518
GGGGTTG318650.016.3396316
GGGGAAT217100.015.28668763
GGGGGAT297350.015.14165634
AGGGGGG635500.015.014564551
GGGTTAA95800.014.51238466
GAGTTAA98150.014.12976666
GGGGTTA119950.014.12148965
TGGGGGG789750.013.98359932
GGGGTGG744300.013.61979916
GTAGGGG164450.013.614037549
GGGGGTG738550.013.41749615
TTATAGG124200.013.38758347
CGCGAAA19350.013.3868111
GTTGGGA187350.013.13118419
Produced by FastQC (version 0.11.2)