FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n02_hbepc_b1_12h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n02_hbepc_b1_12h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36819690
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG180450.030.7595850
GGGTTAA122650.023.4303566
GGGGTTA159850.022.48828565
ATAGGGG89900.019.8770249
TATAGGG81900.018.99651148
GTTAAAA245500.018.07081668
GAGTTAA96750.017.82872666
GGGTTTA167450.017.82100566
GGTTTAA153100.017.19295967
GGGTTGG334650.017.17683817
GGTTGGG345550.016.71466618
GGGAGTT193350.016.70063264
GGAGTTA114650.016.70014265
GGTTAAA164800.016.64221867
GGGGTGT384300.016.62629536
GTAGGGG185500.016.51392449
TGGGGGA479150.016.266420
GTTTAAA218800.015.8455468
GGGGTGG1015150.015.50544116
TGTAGGG176900.015.40566548
Produced by FastQC (version 0.11.2)