FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n02_hbepc_b1_12h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n02_hbepc_b1_12h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37894085
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG261850.044.5293750
TATAGGG145100.039.98904448
ATAGGGG154100.039.1981849
TTATAGG160150.029.85511247
GGGTTGG359150.028.12893317
GAATTAA202450.027.00930466
GGGGTTG373900.026.9085416
GGAATTA169400.026.57657465
GGGGGGT850650.026.03515814
GGTTGGG388500.025.84374618
GTTGGGA240550.025.01495619
GGGGAAT321050.024.08071563
GGGGGTT438200.022.92857615
GGGGGAT375550.022.49801334
GGGAAAA551100.021.1853522
TTTATAG212000.021.03996846
GGGAATT269300.020.27899764
AATTAAA339050.019.8007267
AGGGGGG893650.019.1738451
TTGGGAA316950.019.0500820
Produced by FastQC (version 0.11.2)