FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n02_a549_24h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n02_a549_24h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40503508
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG334850.049.30644250
ATAGGGG271600.043.51498849
TATAGGG267150.043.2842748
GTTGGGA379050.043.2097519
GGGTTGG506050.041.9928417
GGGGTTG526400.040.31701316
GTTTGGG429500.038.66162531
TTATAGG269950.038.20930547
GGGGGTT563450.037.35976415
TAAGGGG272500.037.3327150
GGTTGGG565100.037.2809318
TATAAGG180350.036.99317648
GGGGGGT957450.036.0421314
TTATAAG168200.034.82514647
GGGGAAT382000.033.8485563
ATAAGGG218100.032.7571249
GAATTAA302450.032.5426266
TTGGGAA526200.031.87308920
GGGGAGA753500.031.069094
GGAATTA237650.030.35432265
Produced by FastQC (version 0.11.2)