FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n02_a549_24h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n02_a549_24h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34249395
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG235600.046.6502550
ATAGGGG192050.040.25418549
TATAGGG194450.039.01228748
GTTGGGA269450.037.3905919
GGGTTGG364400.037.27732517
GGGGTTG380150.035.71473316
TTATAGG203700.033.4472347
GTTTGGG318250.033.2812131
GGGGGTT407500.032.80966615
GGGGGGT696850.032.71193314
GGTTGGG412250.032.56515518
TAAGGGG186950.031.36945350
TATAAGG126700.029.8685548
GGGGAAT283350.028.465763
GAATTAA232050.027.10231266
TTGGGAA379600.027.03990220
GGGGAGA549000.026.9573574
TTATAAG119400.026.95637147
ATAAGGG152550.026.68414549
GGGAAAA674250.026.53678522
Produced by FastQC (version 0.11.2)