FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n02_a549_12h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n02_a549_12h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39523737
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG260700.046.8293750
ATAGGGG189800.038.74982549
TATAGGG202450.035.25510448
GTTTGGG335350.031.50174131
GGGGGGT689850.031.4035214
GGGTTGG356400.030.7660217
GGGGTTG362250.030.26917816
GTTGGGA237050.029.15393819
TTATAGG225600.028.36508847
GGGGAAT275700.027.9919263
CGCGAAA27600.025.904551
GGGGGTT422900.025.65890515
GGTTGGG423650.025.60510818
GAATTAA232250.024.53905566
GGGGGAG722450.023.8197083
GGAATTA189150.023.21803965
GGGGAGA505800.022.9034234
GGGAATT246400.021.8837564
GGGAAAA619800.020.60179522
TTTTATA404050.020.07231145
Produced by FastQC (version 0.11.2)