FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n02_a549_12h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n02_a549_12h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44302491
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG251700.043.17243650
ATAGGGG201350.038.8057249
TATAGGG207550.036.88194348
GTTGGGA322050.034.34826319
GGGTTGG419700.034.33918817
GGGGGGT693800.033.875214
GGGGTTG430250.033.5211916
GGGGGTT431550.033.02844215
TTATAGG225550.030.40554447
TAAGGGG218950.029.9513550
GGTTGGG478150.029.91021318
GTTTGGG370700.029.3446331
TATAAGG159650.028.4142448
GGGGAAT303350.026.89595463
GGGGAGA603750.026.7929744
GGGGGAG759150.026.0219733
TTATAAG155500.025.26809747
ATAAGGG191000.024.5812549
GGGAAAA804050.024.40260922
TTGGGAA473050.024.14191420
Produced by FastQC (version 0.11.2)