FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n01_hbepc_b2_12h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n01_hbepc_b2_12h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43808195
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG214650.065.2699241
CGCGTAT126250.063.67002544
TCGCGTA129150.063.33563643
ATCGCGT151350.063.23172442
ACACGTC859850.060.91444413
ACGTCTG875200.059.74765815
CACTCCG875300.059.33071531
ACTCCGG877250.059.218532
AATCGGG343750.059.19336741
CGTCTGA886400.058.9894416
AGAATCG600200.058.44542339
CACACGT899250.058.3030612
CACGTCT912350.057.3750914
GTCACTC907800.057.1843529
CTCCGGA913050.056.8814733
TCCGGAG914550.056.7466834
CGGAGAA919850.056.46821636
GAATCGG364300.056.24256540
GATCGGA926900.055.985751
GCACACG939050.055.90920611
Produced by FastQC (version 0.11.2)