FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n01_hbepc_b2_12h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n01_hbepc_b2_12h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45566059
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5166221.1337868829077362TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG592450.065.71445545
CGTATGC599350.064.9464146
CTCGTAT586100.064.26617444
TATGCCG619050.062.8070448
TTACTCG621450.062.45379635
TACTCGA625250.062.1513436
TCTCGTA609450.061.6454343
ACTCGAT648800.059.8634837
ATCTCGT634250.059.24052442
ACACGTC663200.059.2351813
GTATGCC657650.059.2204647
ATTACTC668200.058.1617234
ACATTAC676450.057.56974432
ATGCCGT677200.057.50051549
CCGTCTT677150.057.47450352
ACGTCTG685150.057.3228115
GCCGTCT686450.056.65060851
CGTCTGA702300.055.8841316
CACACGT705450.055.74133312
TGCCGTC699750.055.64282650
Produced by FastQC (version 0.11.2)