FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n01_hbepc_b1_24h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n01_hbepc_b1_24h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43940678
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4211130.9583670966569974TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG471250.065.2137945
CGTATGC476300.064.50786646
CTCGTAT471150.063.7704844
TATGCCG485200.063.24646448
TCTCGTA492150.060.9512643
GTCACGA518100.059.63702829
AGTCACG530600.058.31017328
ATCTCGT519850.057.75001542
CGAGATT536650.057.45962533
GTATGCC538200.057.10792547
TCACGAG544950.056.75559630
ATGCCGT543350.056.5667749
ACGAGAT552850.055.8445432
ACACGTC558400.055.79000513
CCGTCTT552500.055.42427452
CACGAGA559600.055.33128731
ACGTCTG572900.054.36033615
GCCGTCT564000.054.32463551
CATCTCG568050.052.80731641
CACACGT591500.052.673812
Produced by FastQC (version 0.11.2)