FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n01_hbepc_b1_24h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n01_hbepc_b1_24h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38511957
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4083161.0602317612683252TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG450250.065.2671645
CGTATGC456500.064.3131246
CTCGTAT457050.063.70735544
TATGCCG465300.063.00774848
TATCTCG473600.061.49566741
TCTCGTA476050.061.1429443
ATCTCGT500850.058.1498442
GTATGCC509600.057.71321547
ATGCCGT509200.057.59609249
ACCGCTC514300.057.1055232
CCGTCTT521950.056.09684452
ACACGTC534300.055.97572713
CGCTCAT526900.055.76626234
CACCGCT535300.054.95548231
GCCGTCT533850.054.8721751
TCACCGC540650.054.40542630
ACGTCTG549700.054.25093515
TGCCGTC549000.053.40814250
CCGCTCA555900.052.9190633
CATTATC551500.052.8344938
Produced by FastQC (version 0.11.2)