FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n01_hbepc_b1_12h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n01_hbepc_b1_12h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36819690
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG202950.065.8708441
TCGCGTA119150.063.9895143
ATCGCGT142800.063.75610442
CGCGTAT117450.063.5351544
ACACGTC807100.060.8605313
CACTCCG799600.060.75415831
ACGTCTG817500.060.00748415
AGAATCG548200.059.82945339
ACTCCGG813450.059.82128532
AATCGGG314850.059.56433541
CACACGT832900.059.0207912
CACGTCT838100.058.5230314
CGTCTGA839250.058.45296516
GTCACTC844800.057.63033329
GAATCGG327750.057.5987940
CGGAGAA845650.057.49426736
TCCGGAG850650.057.22542634
CCGGAGA851450.057.1959735
CTCCGGA853450.056.97292333
GATCGGA863400.056.409781
Produced by FastQC (version 0.11.2)