FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n01_hbepc_b1_12h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n01_hbepc_b1_12h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37894085
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5107131.3477380440773277TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG577450.065.90445
CGTATGC584450.065.1264746
CTCGTAT566550.064.8275344
TATGCCG591050.064.35837648
TACTCGA606200.062.7101936
TTACTCG607150.062.57797235
TCTCGTA588100.062.2701843
ACTCGAT622800.060.91131637
GTATGCC635500.059.91111847
ATCTCGT614350.059.5982642
ATGCCGT641950.059.31453749
ATTACTC645050.058.98142634
ACACGTC653500.058.8845713
ACATTAC652000.058.4425932
CCGTCTT651650.058.3683252
GCCGTCT658000.057.83634651
ACGTCTG666700.057.66719415
CTCGATC639200.057.29214538
CACACGT677500.056.7782512
TGCCGTC675400.056.39223550
Produced by FastQC (version 0.11.2)