FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n01_a549_24h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n01_a549_24h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40503508
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7234911.7862428113634008TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG800050.066.6742945
CGTATGC804550.066.254246
CTCGTAT788000.066.2272144
TATGCCG814150.065.45170648
TCTCGTA805950.064.533943
GTCACGA843300.063.5170929
AGTCACG850100.063.01314528
ATCTCGT829550.062.70628442
GTATGCC853050.062.4913847
ACGAGAT863200.062.0645432
TCACGAG865300.061.94995530
ATGCCGT865800.061.57126249
GCCGTCT867300.061.4609551
ACACGTC884250.061.30134613
CACGAGA879500.061.01634631
CATCTCG853650.060.93193441
CGAGATT883700.060.61696633
ACGTCTG895550.060.4669615
CCGTCTT887700.059.97117652
CACACGT912250.059.43100712
Produced by FastQC (version 0.11.2)