FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n01_a549_24h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n01_a549_24h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34249395
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5002331.4605601062442126TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG548750.065.8803645
CGTATGC549650.065.6846246
CTCGTAT551250.065.1247344
TATGCCG558100.064.7024848
TATCTCG565150.063.51695641
TCTCGTA567700.063.21332643
ATCTCGT584800.061.3708242
GTATGCC590750.061.10893247
ACACGTC611150.060.2608413
ATGCCGT602450.059.92206249
ACCGCTC605750.059.6817532
GCCGTCT606800.059.49853551
ACGTCTG621450.059.1960615
CGCTCAT611100.059.1590834
GTCACCG614450.058.9155829
CACCGCT616500.058.70839331
CCGCTCA623400.058.03611433
CACGTCT635950.057.88933614
CACACGT637300.057.8534912
CCGTCTT623550.057.85046852
Produced by FastQC (version 0.11.2)