FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n01_a549_12h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n01_a549_12h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39523737
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG148350.064.4175141
CGCGTAT89500.062.40723844
ATCGCGT106400.061.76814342
TCGCGTA92400.061.3073243
ACACGTC604250.059.33316813
ACGTCTG605050.059.11298415
CACACGT637700.056.2588312
ACTCCGG632850.055.91007632
CACTCCG635050.055.67292431
CACGTCT653350.054.83724614
TCCGGAG649850.054.51648734
AATCGGG256650.054.23929241
GTCACTC653000.054.1953929
GCACACG663450.054.08563211
CTCCGGA657850.053.9007233
CGTCTGA665150.053.8550616
CGGAGAA658900.053.77293836
AGAATCG448300.053.17665539
GAATCGG265500.052.7691640
TATGCGT54550.052.11297258
Produced by FastQC (version 0.11.2)