FastQCFastQC Report
Tue 5 Dec 2017
H5FMCBGX5_n01_a549_12h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FMCBGX5_n01_a549_12h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44302491
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4864871.0981030389465007TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG553750.065.5470845
CGTATGC556000.065.2694246
CTCGTAT544800.064.3252144
TATGCCG569250.063.6955748
TCTCGTA560900.062.3067543
TACTCGA595600.060.8025936
TTACTCG598150.060.49730735
ATCTCGT581350.060.1446742
ACTCGAT604700.059.8247637
GTATGCC613700.059.1102547
ACACGTC630550.058.26413713
ACGTCTG634950.057.80677415
CCGTCTT634250.057.1897352
ATGCCGT636350.057.00629849
GCCGTCT637700.056.90738351
ACATTAC647200.056.12555732
CTCGATC628300.055.60640338
ATTACTC654250.055.43639434
CACACGT666250.055.20946512
TCGATCT636900.055.12091439
Produced by FastQC (version 0.11.2)