FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_nlp7_4tu_pdex_nopd_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_nlp7_4tu_pdex_nopd_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28601686
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1455900.5090259364430474No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG1138380.39801150183943707No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG1046590.36591898813237794No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT982500.3435112181848301No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA498700.17436035064506336No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA458690.1603716648032567No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG428770.14991074302403012No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC414730.1450019414939385No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT409050.1430160445786308No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA393700.1376492280909594No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC389920.13632762767901166No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG385790.13488365685855022No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT373040.13042587769126618No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA368570.12886303275967717No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG367260.1284050178020974No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC362600.1267757432201724No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC358920.12548910578208572No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC358420.12531429091278046No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT353700.12366403854653883No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT350950.1227025567653599No Hit
GCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAG334780.1170490438920279No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC331520.11590925094415762No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA326360.11410516149292736No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC322700.11282551664961288No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC319650.11175914594685082No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT313060.10945508596940753No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG312040.10909846363602481No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT308540.10787475955088802No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC295150.10319321735089322No Hit
GTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCA291900.10205692070040906No Hit
GCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACC290060.10141360198136572No Hit
ACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAG286070.10001857932430977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT207000.035.349551
CTGACTC229400.031.7733522
CTAGCAG234500.031.3938268
ACTCTAG234950.031.1704795
GACTCTA242500.030.4741694
TGACTCT264750.027.767693
CTCTAGC271000.027.3596086
TAGCAGA273400.026.991039
CTATCGA316500.023.06144317
GTATGCC37550.022.7479547
CGTATGC37200.022.67977546
TCTATCG321450.022.6733516
TATGCCG39800.021.63816648
GATCTAT340850.021.48551614
ATCTATC343200.021.3791415
AGCAGAT362550.020.36362810
TATCGAT363650.020.16785418
CCCTACA36000.020.11909710
AGATCTA366550.020.05556713
GCAGATC371750.019.80323411
Produced by FastQC (version 0.11.2)