FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_nlp7_4tu_pdex_nopd_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_nlp7_4tu_pdex_nopd_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25830718
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1375990.5326952196992744No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1098260.4251759474901163No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG1094690.4237938720867147No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG948710.3672797635745162No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA473540.18332436597387652No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG451290.17471059070057596No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC450710.17448605183951912No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC417500.16162926636417926No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC415000.1606614264458309No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG414340.1604059167073869No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT407540.15777339212947933No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC400610.1550905398758176No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA395220.1530038770118585No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA394810.1528451512652494No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT391720.1516489011261708No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC384670.14891959255642837No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT384010.1486640828179844No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA378020.14634513837362167No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT372770.14431267454509009No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC352510.13646929984679482No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG348460.13490139917907043No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG337110.1305074059497688No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA334300.12941955388154522No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC333940.12928018493330304No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG332250.12862592514849955No Hit
GCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAG331370.12828524549724094No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG328900.1273290196579127No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC328430.12714706575326323No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT326330.1263340802218506No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT312730.12106903106603542No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC307530.11905592403587079No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC292610.11327985540316766No Hit
GCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCC289610.11211844750114959No Hit
GCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACC286560.11093768280076458No Hit
GTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCG268980.10413183249493878No Hit
TTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCC267670.10362468437772424No Hit
GGTACATCCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATT258890.10022563058448472No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG258830.10020240242644435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT202100.039.897831
CTGACTC237200.034.164942
ACTCTAG244000.033.5565
CTAGCAG245450.033.2288258
GACTCTA247400.033.1939054
TGACTCT262800.031.0757273
CTCTAGC275750.029.717616
TAGCAGA278600.029.4006449
CTATCGA320400.025.22683317
TCTATCG329150.024.65160416
GATCTAT341250.023.83884414
AGCAGAT349150.023.5901210
ATCTATC345650.023.54564315
GCAGATC360950.022.69290511
AGATCTA363850.022.56016213
TATCGAT367950.021.9672518
CCGGGGT17500.020.8073581
GCGGGGG28650.020.2864061
TTCTAGC409600.019.74416524
CCCTACA28900.019.73446810
Produced by FastQC (version 0.11.2)