FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_nlp7_4tu_mdex_nopd_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_nlp7_4tu_mdex_nopd_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28376867
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1243230.43811390454062454No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG1005880.35447183087548034No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT892350.31446389060497765No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG877470.3092201827636574No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA413390.145678520465279No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA385860.13597695615939562No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC380440.13406694967418353No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG357340.1259265161301986No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC342510.12070042827490433No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA339080.11949169723352475No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT329670.11617561586344258No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC325640.11475544498975168No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT320910.11308859431169763No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG309970.10923334136922164No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC309560.10908885748380892No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA304220.10720704297623836No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT302840.10672073136192237No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG300150.10577277611372672No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC295110.10399668152231183No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT291430.10269985055080252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT187100.034.8449971
ACTCTAG217750.030.3875875
GACTCTA220450.030.2376274
CTAGCAG219950.029.8601828
CTGACTC220500.029.6604752
TGACTCT251800.026.3061753
TAGCAGA252350.026.0540249
CTCTAGC258550.025.70066
CTATCGA286700.022.67639217
TCTATCG296850.021.99502416
GATCTAT316250.020.66774614
ATCTATC318500.020.44491215
AGCAGAT340800.019.46653210
AGATCTA344000.019.2040413
GCAGATC349000.018.99909211
TATCGAT345250.018.96268718
GTGCGCA385850.018.43796568
TGCGCAT387750.018.42839469
CTAGCAT353100.018.34440826
TAGCATG354100.018.29211827
Produced by FastQC (version 0.11.2)