FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_nlp7-1xe1728_rep5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_nlp7-1xe1728_rep5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32824028
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGATCTATGTTTCGAGTTATTTGGTGATAGGGTAAAGAACTGGATCA367940.11209471305593574No Hit
GGCGTATTGAGTGACGTAATAGTTGAGACCAAGAAAATCATATGAACCCT367750.11203682863053857No Hit
GTCCAATCATACCTTTTTGGTCATCCTTATATTTCGTCCTGTAAACATCA350640.10682418379608988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC41600.022.63594444
TATGCCG41750.022.30379146
GTATGCC46650.020.48581745
CGCGGGG17000.020.3901251
GTCCGCT24550.018.8259141
CCCTACA38250.018.02109710
ATGCCGT53250.018.01307347
GCGGGGG34050.016.7611621
GGCCCGC8150.016.3252431
GCCGGGT58500.016.2198581
CTACACG41250.015.77737512
CGGGCAT25750.015.5010561
TCCCTAC47700.015.3311359
GTCCGCG11400.015.0495971
TGCCGTC62500.014.95553848
TCGTATG63500.014.88377843
GCGGGCC10600.014.8641581
CTCGTAT64400.014.67577442
TAGGGTA203750.014.39176431
GCGGGGT27750.013.8791651
Produced by FastQC (version 0.11.2)