FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_nlp7-1xe1728_rep4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_nlp7-1xe1728_rep4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38008998
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTATTGAGTGACGTAATAGTTGAGACCAAGAAAATCATATGAACCCT455210.11976374646866512No Hit
CGCGGATCTATGTTTCGAGTTATTTGGTGATAGGGTAAAGAACTGGATCA422330.11111316325676357No Hit
GTCCAATCATACCTTTTTGGTCATCCTTATATTTCGTCCTGTAAACATCA401960.10575390595668951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG15850.025.1824741
CCCTACA43450.022.06512510
CTACACG44250.020.63824812
TCCCTAC49600.020.2463369
GTCCGCT30150.017.3030151
GCGGGGG44500.016.4440611
TACACGA53750.016.2744613
CGTATGC39900.016.23047844
TATGCCG42050.015.0681946
GCGGGGC13150.014.91025451
TAGGGTA235900.014.59646331
GCCGGGT82650.013.9372091
CGGGGCC14900.013.8639991
CCTACAC72250.013.85076611
GCGGGAC19650.013.541721
TATTTCG244800.013.507771530
GTATGCC48300.013.48049145
CCTTATA247700.013.46405725
GATAGGG253500.013.44459629
CGGGCAT36050.013.208591
Produced by FastQC (version 0.11.2)