FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_nlp7-1xe1728_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_nlp7-1xe1728_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27723557
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTATTGAGTGACGTAATAGTTGAGACCAAGAAAATCATATGAACCCT333440.1202731669677163No Hit
CGCGGATCTATGTTTCGAGTTATTTGGTGATAGGGTAAAGAACTGGATCA302150.10898673644222494No Hit
GTCCAATCATACCTTTTTGGTCATCCTTATATTTCGTCCTGTAAACATCA295990.10676479933653535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG12200.026.4035531
CCCTACA33550.022.11003310
TCCCTAC37650.020.817569
GCGGGGG27800.018.8921261
CTACACG36250.017.76049612
GCGGGCC11450.017.4302541
CGTATGC27900.016.43636944
GCGGGGC8950.016.4308741
TACACGA40800.016.38014213
TATGCCG29100.015.87924846
GCCGGGT46700.015.1449861
CGGGGGG21100.014.7686891
GTCCGCT19900.014.6035821
TAGGGTA172750.014.5842231
CCTACAC52400.014.35665411
CGGGGCC11200.013.7552661
TTCCCTA62900.013.6289218
CGGGCGT28200.013.409391
GATAGGG188450.013.38761329
TATTTCG185950.013.02179430
Produced by FastQC (version 0.11.2)