FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_nlp7-1xe1728_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_nlp7-1xe1728_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39083993
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTATTGAGTGACGTAATAGTTGAGACCAAGAAAATCATATGAACCCT414490.10605108848525278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG14900.026.0832731
CCCTACA43450.020.21266710
CTACACG48750.017.15394812
TCCCTAC54150.016.5417829
GCGGGGC12750.016.4765551
TACACGA54850.014.86348513
CGGGGCC12850.014.7134991
GCGGGCC11950.014.6496571
TAGGGTA216200.014.63120931
GCGGGGG42050.014.571271
GTCCGCT28100.014.3290341
CGGGGGG25200.014.1717991
GGCCCGC10800.013.9402331
CGGGCAT38150.013.3993481
GCGCATA138200.013.34924746
GCCACGG138900.013.25829770
GATAGGG238100.013.07955829
GCCGGGT86000.013.06868651
TGCGCAT145100.012.93124845
TGCCACG140750.012.86017669
Produced by FastQC (version 0.11.2)