FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_nlp7-1xe1728_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_nlp7-1xe1728_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28237512
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTATTGAGTGACGTAATAGTTGAGACCAAGAAAATCATATGAACCCT296640.10505174818518007No Hit
CGCGGATCTATGTTTCGAGTTATTTGGTGATAGGGTAAAGAACTGGATCA284500.10075250255759077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA32300.023.94092210
TCCCTAC38650.021.1845389
CTACACG35100.019.53886212
CGCGGGG12600.019.1715551
GCGGGGC10700.018.322431
GTCCGCT22050.016.8296891
TACACGA42350.016.2765813
GCGGGGG36200.015.1834291
GCGGGCC9600.014.2223771
TAGGGTA169050.014.07543331
CCTACAC55550.013.98360811
CGGGCAT28950.013.4231121
GCCGGGC11050.013.30656051
GCGCATA95000.013.11834446
TTCCCTA66800.013.0429418
GATAGGG180450.012.95346829
GCCACGG94800.012.81510770
TGCCACG95850.012.78430469
ACACGAC53100.012.71767614
CGGGGCC10350.012.5152691
Produced by FastQC (version 0.11.2)