FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_e1728_rep5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_e1728_rep5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23377314
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGATCTATGTTTCGAGTTATTTGGTGATAGGGTAAAGAACTGGATCA276330.11820434118308032No Hit
GGCGTATTGAGTGACGTAATAGTTGAGACCAAGAAAATCATATGAACCCT276070.1180931222466362No Hit
GTCCAATCATACCTTTTTGGTCATCCTTATATTTCGTCCTGTAAACATCA257900.1103206296497536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA24800.022.71488210
CGCGGGG11550.020.9161241
CGTATGC22350.019.57830244
GTCCGCT17750.019.1331941
TCCCTAC31750.017.9630539
GCGGGGG25250.017.4712221
TATGCCG24600.017.21928646
CTACACG28750.016.79486812
GTATGCC26900.015.87670445
CGGGGCC9450.014.8197991
CGGGCAT22100.014.7334611
CCTACAC39500.014.52723811
TAGGGTA159500.014.43510931
TACACGA34300.014.2813213
GTCCGCG8700.014.08519651
GGGGTCT18000.014.004711
GCGGGGT21200.013.8725891
GCCGGGG32400.013.5076291
ATGCCGT32550.013.44388547
GATAGGG172600.013.44067629
Produced by FastQC (version 0.11.2)