FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_e1728_rep4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_e1728_rep4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25932992
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGATCTATGTTTCGAGTTATTTGGTGATAGGGTAAAGAACTGGATCA267450.10313117745919945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA30100.024.87646110
CTACACG34000.020.68517912
GTCCGCT21850.020.1897131
TCCCTAC38900.019.3389059
TACACGA39950.018.83062213
CGCGGGG12400.017.5057621
GCGGGGC10500.017.00561
TATGCCG27700.015.29210446
TAGGGTA153800.014.76552731
CGTATGC28350.014.570394544
ACACGAC49350.014.10922214
CCTACAC57000.014.0573311
GCGGGAC14500.014.004611
GCCGGGC15950.013.8290051
CGGGGCC9900.013.792421
GATAGGG166000.013.42711629
GTATGCC32450.013.37709845
GCGGGGG35750.012.633531
TTCCCTA70050.012.3875358
GCGGATC231800.012.3632052
Produced by FastQC (version 0.11.2)