FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_e1728_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_e1728_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29525193
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA29450.020.7925710
CGCGGGG14000.017.5056021
TCCCTAC40750.016.4865239
GTCCGCG10200.015.7893681
GCGGGGC12200.014.9228091
CTACACG36550.014.743175512
GCGGGGG41300.013.9027551
GTCCGCT24700.013.8910851
TAGGGTA159700.013.78239631
GCGCATA89850.013.558475546
TACACGA41900.013.27822613
TGCGCAT94000.013.22032445
TGCCACG93900.012.82590969
GCCACGG95900.012.70455970
TTGCGCA99800.012.38154144
GATAGGG176650.012.24179329
CGCATAA102000.011.94350147
GGGCACT21700.011.7779631
GTTGCGC108750.011.74839943
CCTTATA164800.011.72153225
Produced by FastQC (version 0.11.2)