FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_e1728_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_e1728_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28457693
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGATCTATGTTTCGAGTTATTTGGTGATAGGGTAAAGAACTGGATCA333820.11730395714086873No Hit
GTCCAATCATACCTTTTTGGTCATCCTTATATTTCGTCCTGTAAACATCA315460.11085227463800386No Hit
GGCGTATTGAGTGACGTAATAGTTGAGACCAAGAAAATCATATGAACCCT315190.11075739695413821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA33400.024.93292410
TCCCTAC39050.022.7591179
TATGCCG29850.020.6406146
CTACACG37350.020.60981412
CGTATGC32450.019.30967344
GTCCGCT21700.018.3934921
CGCGGGG13700.017.3783041
TACACGA44550.017.12188113
GTATGCC36050.016.9935745
CCTACAC52550.016.51283611
GCGGGGG28750.016.0751551
ATGCCGT38550.015.98252947
TTCCCTA65600.015.094738
TCGTATG42250.014.74721543
TAGGGTA194400.013.89582731
ACACGAC55950.013.75819714
CTCGTAT46100.013.59154642
CGGGGGG20050.013.0968221
GATAGGG209400.012.74985129
GCGGGGC11050.012.6740891
Produced by FastQC (version 0.11.2)