FastQCFastQC Report
Thu 23 Jun 2016
H5FM3BGXY_n01_e1728_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FM3BGXY_n01_e1728_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33120171
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA37550.020.68640910
TCCCTAC45150.018.1342749
CTACACG41450.017.8114812
CGTATGC33550.017.21615444
TATGCCG34500.016.64089646
CGCGGGG18700.015.9150231
TACACGA47300.015.90459613
GTATGCC39550.014.4273745
GCGGGGC17100.013.923321
TAGGGTA205200.013.18129331
GCGCATA95550.012.89626446
ATGCCGT46100.012.83335847
GCGGGGG50050.012.8019751
TGCGCAT100950.012.62237545
GTCCGCT26300.012.5141721
CCTTATA212850.012.4787725
TATTTCG211500.012.30885830
CCTACAC71600.012.26599311
GGGGTCT25550.012.1963281
TGCCACG104700.011.90442469
Produced by FastQC (version 0.11.2)