FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7269739
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG247650.3406587224108046No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC82350.11327779442975876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG172100.037.03153670
AAAAGGG390900.034.9979570
CTAGATC52450.030.9631425
CATTAAA547250.029.8725861
TCATTAA540750.029.37732760
CGAGATC45500.029.0772255
GCAGATC50750.028.9684054
TGTCGGC27050.028.20518130
GTATCAT520050.028.12392457
CGTATCA548600.028.0572556
CCGTATC547250.027.95359655
CGCCAAG12700.027.881
ATCATTA566950.027.81602359
CGAGCAG14950.027.4359361
TATCATT546550.027.17653758
TTAAAAA697350.027.06626163
CGCCGTA596650.026.93604753
GCCGTAT620250.026.6727354
CGCGTAG3550.026.6412492
CAGATCG125250.026.5471785
Produced by FastQC (version 0.11.5)