FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7948769
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG216610.2725076046366425No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG128430.16157218809604357No Hit
CGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAAAC100390.12629628562611392No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC93900.1181314993554348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC31350.026.23435230
CCGTATC121700.023.29377755
CGCCAAG17650.023.0403351
GTGGGAC36800.023.01539819
CGAGCAG17400.022.7669411
CGTATCA125100.022.66055156
TCGGCGG36800.022.34924132
CGGCGGC37200.022.2030133
GTCGGCG37900.021.88513231
CGCCGTA132500.021.76518253
CGCACAG7650.021.5383261
GCCGTAT144000.020.85334854
GTATCAT130750.020.66444857
TCGCCGT137200.020.66245352
CGGGAAG100300.020.586851
GTGCAGG39100.020.22925614
CGGCAAA78200.020.1286721
CGGCAAG20950.019.913081
CGCGAAG11100.019.8972511
CGCGAAT9000.019.865671
Produced by FastQC (version 0.11.5)