FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5767407
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236170.4094907815591998No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC106660.18493579523692363No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG97250.16861997081183971No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA91940.15941306032329605No Hit
CGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAAAC59870.10380748228796753No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC33300.032.68604330
AAAGGGG87100.029.89516670
GTGGGAC36850.029.34774419
CGGCGGC37700.028.68580833
GAATGTC38250.028.36458627
CGGCAAG11050.028.2302341
CGAGCAG12700.028.1503141
TCGGCGG39000.027.72961432
AAAAGGG173000.027.59398870
CATTAAA220900.026.98144161
TCATTAA217900.026.63014460
CGGCCTA1455.875336E-1026.5895771
CGTATCA208950.026.5818156
CCGTATC207750.026.4491955
GTATCAT202600.026.29209557
CGCGCAG2450.025.7509461
CGAGATC20000.025.5509325
AATGTCG42700.025.4905228
CGCCGTA228850.025.31050553
CGCGGAG4750.025.0883481
Produced by FastQC (version 0.11.5)