FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8665590
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG305460.3524976372064683No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG22600.025.2850131
TGTCGGC22050.024.60196330
AAAGGGG107200.023.50616870
CCGTATC189200.022.1975155
CGTATCA191600.022.15692156
CGAGCAG19050.022.0836221
CGCCGTA201950.021.5933953
CATTAAA219100.021.13314461
CGGGAAG68550.021.0193041
TCGCCGT206650.020.93303552
GCCGTAT215100.020.92411854
GTATCAT196550.020.53054257
CGGCGGC26600.020.52530333
TCATTAA218750.020.3508860
GTCGCCG245750.019.99506251
GGTCGCC250300.019.91112350
CGCGAAG13400.019.8834971
CGCGAAA14450.019.6517471
GTGGGAC28900.019.6184119
TGGTCGC237300.019.46806549
Produced by FastQC (version 0.11.5)