FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4307434
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG124590.2892441300319401No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAG1103.142486E-828.6803551
TGTCGGC11900.027.35139330
CGAGCAG10400.026.9644371
CGGCAAG10950.025.9301871
AAAGGGG43150.024.33234470
CGCGAAG5400.024.0183241
CGCGGAG3850.023.6726741
CCGTATC99800.023.6712855
CGTATCA100500.023.57605656
CGCCGTA109950.022.95057753
CATTAAA111050.022.72297761
CAGATCG24800.022.7212815
TCGCCGT112250.022.13734652
GCCGTAT117050.022.0667654
CGGCGGC14750.022.06654733
GTATCAT102500.022.05754557
TCATTAA111750.022.01690960
AAAAGGG96700.021.96876770
CGGCCTA1304.2920055E-621.571551
CGCGAAA6550.021.4068831
Produced by FastQC (version 0.11.5)