FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5713893
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200950.3516866696663728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG102500.032.64211370
AAAAGGG234150.032.25533370
CTAGATC28750.030.801285
GTGCGTC4600.030.4835341
TGTCGTG23250.029.5526391
CGAGATC25400.027.8357985
TGTCGGC16150.027.73817630
AAGCGTC19850.027.197151
TGCGTCG15300.026.1202051
CGAGCAG11750.025.9564061
CAGATCG62400.025.8023535
CATTAAA364100.025.40509261
GCAGATC31850.024.729334
TCATTAA367500.024.65579460
CGCGAAA7450.023.998111
CGCGCAG2200.023.9018631
CAAGATC49700.023.24045
ATCATTA391150.023.0039859
TTAAAAA462200.022.6253363
CGGCAAG12150.022.5051271
Produced by FastQC (version 0.11.5)