FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4075461
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG135640.33282124402613594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG8400.030.0506881
CCCGGCG2500.027.99878716
AAAGGGG59550.026.85829270
CGGCAAT7600.026.7556571
CGAGCAG7850.024.1171131
CAGCCCG3300.023.33232313
TGCGTCG3100.022.6187951
CGCCAAG7650.022.4561791
CCGTATC104100.022.18951255
CGTATCA104700.022.0955156
AGCCCGG3200.021.87405214
CGCCGTA115000.021.82106253
CGCGAAA4500.021.8145731
GCGAGCG6800.021.61671311
CGGGCAG3250.021.5748541
CCGGCGG3250.021.5375317
GGCGAGC6900.021.30342710
TCGCCGT116400.021.22786352
CGACAAG9250.021.224991
CGCGGAG3800.021.2200031
Produced by FastQC (version 0.11.5)