FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485801
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAA308.274557E-658.4496351
CGCGAAA806.130176E-835.069781
CCCATGG450.00430532431.11216246
CGCCAAG1151.4260877E-930.495461
CGCTGGC500.00720876727.99806665
CGGCAAC1403.8562575E-1027.5548271
CAGCCAG906.0711973E-627.2764931
CGCCAAC906.0711973E-627.2764931
CGGCAAG1705.456968E-1226.8180661
TGTCGGC1606.548362E-1126.24818630
CGCACAG750.001810411223.3798521
CGCCCAA750.001810411223.3798521
GGAGTGC1403.2810385E-722.49844657
GCGATGT1102.8927852E-522.298732
CGGCGGC1905.620677E-1022.10373533
TCGGCGG1905.620677E-1022.10373532
CGGCAAA4000.021.9186121
GCGCAAG800.002634309221.9186121
GGCAACA2401.8189894E-1221.9005412
CCTGTCC800.002664428421.8757441
Produced by FastQC (version 0.11.5)