FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993564
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41950.21042715458344952No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAG4800.032.136491
CGCGCAG901.734461E-731.1626511
CGCGGAT1151.4370016E-930.4852031
CGGCAAG4900.028.6187631
ACGCGCC1403.965397E-1027.4985838
TGCGGTG3450.026.4205111
CGCGGAG1551.2678356E-924.879861
CGCCAAC2850.024.6020971
GTACGCG1151.4994221E-624.3465760
TGTCGGC5800.024.13668630
CGCCCAC901.9080401E-423.3719881
CGGCAAC4200.022.5372771
CGGGCAG1403.2373828E-722.5372771
CGCGAAT1604.8301445E-821.911241
TGCGTCG1604.8301445E-821.911241
CGCCAAG3850.021.8543281
CGCCAAT3900.021.5741461
CGGCCAG1807.1395334E-921.4243221
GCGGCCT2351.6370905E-1120.84998923
CGCCAAA7100.020.7385271
Produced by FastQC (version 0.11.5)