FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5798108
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195550.3372651906449483No Hit
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG99870.17224584295428785Illumina Single End PCR Primer 1 (100% over 32bp)
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT78210.13488882925257686Illumina Single End PCR Primer 1 (100% over 31bp)
CGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG77990.13450939513372293Illumina Single End PCR Primer 1 (100% over 30bp)
CGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGA61720.10644851734393358Illumina Single End PCR Primer 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGTG29550.039.2741931
GTGCGTC5000.036.4644851
ACGCGTC3050.033.3376461
AAAGGGG97450.030.77804470
CGAGATC26550.030.5838185
AAGCGTC25900.030.0531461
AAAAGGG210600.029.44742870
CTAGATC30050.028.6522585
CGGCAAG11250.028.3612691
CAGCCCG12050.028.17234413
CGCGCAG2250.028.0496041
CCCGGCG12500.027.15860716
TCGCGTA3650.026.8968811
TGCGTCG19800.026.7392081
CGTCGTA10000.026.6471251
CCGGCGG12850.026.14651917
GCGGCCT13600.025.73308823
CGCGTAG3550.025.659712
GGCGGCC13800.025.61396622
AGCCCGG13300.025.52478814
Produced by FastQC (version 0.11.5)