FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5809029
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174790.3008936605412023No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCC2000.034.9985466
CGAGATC13600.026.508075
CGCGAAG6050.024.91661
TGTCGGC13150.024.48524730
ACCCGCG1151.5002315E-624.34659861
CCGTATC134050.024.12390555
CGTATCA134500.024.09523256
CGGCAAG10150.023.8319051
GCGAGCG10550.023.55331411
CGCCGTA145300.023.48470953
CGCGAAA7050.023.3713861
AAAGGGG67900.023.19490470
TCGCCGT147850.022.89029152
GCCGTAT156050.022.29301354
GGCGAGC11350.022.20152510
TTAACCG18400.022.06410458
GTCGCCG174350.021.90024451
TGCAGTT23100.021.8537641
AAAAGGG118600.021.83719870
TGCAGTG11150.021.6945151
Produced by FastQC (version 0.11.5)