FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4293081
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG154170.3591127211436262No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG47860.11148170742643804No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA5450.026.3700351
AAAGGGG49150.026.34651870
TGTCGGC13650.026.15215530
AAAAGGG100850.023.21641770
CGGCAAG10950.022.728331
TGTCGTG11800.022.2793541
CGCGCAT1755.173206E-922.0332181
CATTAAA129400.021.71857561
CGCGGAG2950.021.388181
TCGGCGG16400.021.3403332
CGAGCAG9700.021.3208051
TCATTAA131250.020.90580260
CGGGAAG42550.020.6774731
CCGTATC126150.020.55800655
CGTATCA127200.020.4158256
GTATCAT122300.020.14634357
CGCCGTA138550.019.88008753
GTGGGAC17450.019.85591119
GTCGGCG17750.019.71726431
GCCGTAT147600.019.37250354
Produced by FastQC (version 0.11.5)