FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4176021
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG165610.3965736762339078No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG64000.028.60005470
CGCACAG5250.026.713881
AAAAGGG125650.026.5167170
CATTAAA176350.025.77975861
CGAGATC16300.025.5520255
CTAGATC16850.025.3411255
TCATTAA176250.025.07952960
CGGCAAG9250.025.0171871
TGTCGGC11250.024.88757930
GGCGGCC4250.024.7045822
CGCGAAA6400.024.6529461
TGTCGTG12250.024.6149331
CGTATCA172850.024.45922756
CCGTATC173250.024.1199455
GTATCAT171150.023.51614457
TGCGTCG6000.023.3746431
GTCCGCA13800.023.07877213
CGCCGTA188700.022.99853353
AGTCCGC14150.022.75525912
ATCATTA194100.022.70101759
Produced by FastQC (version 0.11.5)