FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5861899
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161880.27615624220069296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAG11700.025.4668331
CGGCAAG15150.023.1381821
CGCGCAG2207.2759576E-1222.3073121
CGCGAAA9750.022.290971
CCGTATC76600.022.2505855
CGTATCA77950.022.17951456
TGTCGGC15650.022.13958230
CGCCGTA81450.021.57054953
CGGGAAG48300.021.5551571
CGGCGGC17000.021.41080533
TCGCCGT82400.021.27956652
GGTCGCC95500.020.63293550
GTCGCCG92850.020.5054551
CGCGGAG5700.020.294621
GCCGTAT89950.020.27129254
CTCGGTG84700.019.95812444
TGGTCGC96650.019.73561549
CGGGCAG4500.019.4746381
CGCCAAT13050.019.340331
TCTCGGT85800.019.25354443
Produced by FastQC (version 0.11.5)