FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4811980
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG178910.3718012128063708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATC15500.027.5471455
TCGCCGG23950.026.5961552
TCTCGGG17050.026.47973843
CGGATCA16800.026.45713656
CCGTGTC11200.026.24881255
GGTCGCC62400.025.96837650
ATCTCGG46250.025.9555842
GTCGCCG62350.025.87720551
CAGATCG27050.025.6201935
CGAGATC13950.025.5923675
GATCTCG47450.025.15165141
CGCCGTA35800.024.83153553
CCGTATC33900.024.7776455
TGTCGGC12450.024.73732230
CGCCGGA20700.024.6851353
GCCGGAT23300.024.63433354
CGTATCA36150.024.59086656
CTCGGTG52100.024.58621844
CGTGTCA12050.024.39723656
TGGTCGC49850.024.36198449
Produced by FastQC (version 0.11.5)