FastQCFastQC Report
Sun 15 Mar 2020
H5FGKAFX2_n02_vk6-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FGKAFX2_n02_vk6-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184383
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98480.2353513050789089No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG45740.10931121744830719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG3400.025.7808071
CGAGCAG9950.025.0190411
CGGCAAG12600.022.818061
CGGGCAG4550.022.3471431
TAGTCCG11850.021.56008111
AGTCCGC12350.021.25397712
GTCCGCA12350.021.25397713
CGCGCAG1859.786163E-920.8476141
TGTCGGC11800.020.76140430
CGGGCTA2453.092282E-1120.035371
CGGGAAG40100.019.760571
GTACGCG3400.019.55782560
CGGCAAA33950.019.1058921
CGCGAAA7250.018.8608841
CGCCAAA16200.018.6131061
CGCCAAT10050.018.4903531
CGCACAG4450.018.121881
CGCGAAG4850.018.0731431
CCGTATC52300.017.86694355
GGGTCTC15000.017.73242861
Produced by FastQC (version 0.11.5)